Fig 3. Effect of ESX-1 disruption (M. marinum eccCb1 Transposon Mutant) on gene transcription during infection (Indicated as ínt’) and growth in culture medium in M. marinum compared to that in the wild-yype strain E11 during growth in 7H9 culture medium.
(A) Relative transcript expression levels of the ESX-1 secretion system-associated genes, including the main esx-1 locus as well as the EspR regulator and accessory factors in the espA operon, which is encoded outside the RD-1 region. (B) Gene expression levels, as measured by qRT-PCR, were compared to those of the wild-type strain E11 grown in similar conditions. Values represent mean ± standard error of the mean of two biological replicates. (C, D) Regulation of genes associated with cell wall synthesis, including genes involved in mycolic acid synthesis (C) and PDIMs (D).