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. 2019 Jan 23;14(1):e0210985. doi: 10.1371/journal.pone.0210985

Fig 1. CCPs internalisation into PC LUVs.

Fig 1

Time-dependent percent of total fluorescence evolution. (A) PC LUVs were incubated with NBD-labelled CPPs for 2.5 hours at 35°C. (B) Quantification of CPPs penetration into LUVs composed of PC and PC/SM/Chol. Means ± SEM of 3 experiments. * Indicates that the P value for RW9 was from 0.02 to 0.04 when comparing RW9 with penetratin and R9 (unpaired t-test). (C) PC LUVs incubated with NBD-labelled CPPs for 5 minutes at 50°C. In A and C dithionite was added (arrow head) to quench the CPPS in solution. After stabilization, melittin was added (two arrow heads) allowing the CPP quenching inside the LUVs. The difference of fluorescence before and after melittin is the percent of internalized peptide (Zoom). Penetratin green, R9 blue and RW9 red.