Table 2. Isothermal Titration Calorimetry of TAGV-Ub binding.

Protein	Na	KD	ΔHb	ΔGc	-TΔSd
	(sites)	(μM)	(kJ/mol)	(kJ/mol)	(kJ/mol)
Ube	1.34 ± 0.09	11.5 ± 2.5	-19.6 ± 1.5	-28.3 ± 0.6	-8.6 ± 2.0
Ub-L8Ae		>1 mM
Ub-L8Ne	0.99 ± 0.14	295.3 ± 39.7	-11.0 ± 2.5	-20.2 ± 0.3	-9.2 ± 2.9

^a Binding stoichiometry.

^b Binding enthalpy.

^c Gibb’s free energy.

^d Entropy factor.

^e Average from n = 3 with error calculated using the standard deviation