Table 1. Primers used in this study for detection of toxin and colonization factor genes in ETEC strains.
| Gene (Toxin/CFs) | Primer sequence (5’– 3’) | Concentration (pmol/uL) | PCR type (Mn/Mt) | Product size (bp) | Reference |
|---|---|---|---|---|---|
| eltB (LT) | F: GCACACGGAGCTCCTCAGT | 0.2 | Mn | 218 | Vidal et al.[24] |
| R: TCCTTCATCCTTTCAATGGCTTT | |||||
| sta2 (STh) | F: TTCTTTCTGTATTGTCTTTTTCACC | 0.2 | Mn | 193 | Vidal et al.[24] |
| R: TAATAGCACCCGGTACAAGCAG | |||||
| sta1 (STp) | F: CCTCGACATATAACATGATGCAACTC | 0.2 | Mn | 127 | This study |
| R: AAATTGCCAACATTAGCTTTTTCA | |||||
| sta1 (STp) | F: TCTTTCCCCTCTTTTAGTCAG | 0.2 | Mn | 166 | Rodas et al.[25] |
| R: ACAGGCAGGATTACAACAAAG | |||||
| cfaB (CFA/I) | F: ACTATTGGTGCAATGGCTCTGAC | 0.2 | Mt1 | 497 | Vidal et al.[24] |
| R: CAGGATCCCAAAGTCATTACAAG | |||||
| cooA (CS1) | F: GAGAAGACCATTAGCGTTACGG | 0.16 | Mt3 | 410 | Vidal et al.[24] |
| R: CCCTGATATTGACCAGCTGTTAG | |||||
| cotA (CS2) | F: ACTGTAACTGCTAGCGTTGATCC | 0.2 | Mt1 | 358 | Vidal et al.[24] |
| R: TGCTTCCTGCATTAATAACGAGT | |||||
| cstH (CS3) | F: CCCACTCTAACCAAAGAACTGG | 0.48 | Mt3 | 300 | Vidal et al.[24] |
| R: CGTATTTCCAGCATTTTTATCCA | |||||
| csaB (CS4) | F: ATTGATATTTTGCAAGCTGATGG | 0.32 | Mt3 | 242 | Vidal et al.[24] |
| R: GTCACATCTGCGGTTGATAGAGT | |||||
| csfA (CS5) | F: TCCGCTCCCGTTACTCAG | 0.2 | Mt2 | 226 | Sjöling et al.[27] |
| R: GAAAAGCGTTCACACTGTTTATATT | |||||
| cssA (CS6) | F: AAATGTATCCCAGGTAACGGTCT | 0.2 | Mt2 | 165 | Vidal et al.[24] |
| R: TGTTGATTAGGCGTAACCTCTGT | |||||
| csvA (CS7) | F: TGCTCCCGTTACTAAAAATAC | 0.16 | Mt4 | 203 | Del Canto et al.[16] |
| R: TAGATGTCGTATCACTACGT | |||||
| cswA (CS12) | F: GCGAATAACAATGATGCAAG | 0.16 | Mt4 | 263 | Del Canto et al.[16] |
| R: CCTGACTGGTTTACAAGATA | |||||
| cshE (CS13) | F: GGGACTGCCACAATGAATTT | 0.4 | Mn | 178 | Sjöling et al.[27] |
| R: CAGCACCACCTGCTGATTTA | |||||
| csuA1 (CS14) | F: TTTGCAACCGACATCTACCA | 0.4 | Mn | 162 | Sjöling et al.[27] |
| R: CCGGATGTAGTTGCTCCAAT | |||||
| csbA-csdA (CS17-19) | F: TAAACTTGATCTTCTGCAAGC | 0.16 | Mt4 | 348 | Del Canto et al.[16] |
| R: GCATGAATCGTAAGCTGTTG | |||||
| csbA (CS17) | F: TAAACTTGATCTTCTGCAAGC | 0.16 | Mn | 324 | Del Canto et al.[16] |
| R: TCAGGCGCAGTTCCTTGTGTG | |||||
| fotG (CS18) | F: ATCCGTCAGGTGTTTGTGGT | 0.4 | Mn | 362 | Rodas et al.[25] |
| R: CACCTGAATTCCTCGACAGG | |||||
| csnA (CS20) | F: AGGTATCCAAATCCGCACTG | 0.4 | Mn | 114 | Sjöling et al.[27] |
| R: CATCAGCCAGCACATAGGAA | |||||
| fotA (CS18) | F: TGGTGTAGGTGTGTTTGTCC | 0.2 | Mn | 193 | This study |
| R: AGTACCAGCTTTAACCTGACC | |||||
| csnA (CS20) | F: CCTGATTAACTGTGACAGCCT | 0.2 | Mn | 189 | This study |
| R: ACAACGTCAAGTTTTTGATCGC | |||||
| lngA (CS21) | F: TCATGAGCCTGCTGGAAGTTATCA | 0.16 | Mn | 617 | Pichel et al.[28] |
| R: TCCGGCTACCTAAAGTAATTGAGT | |||||
| csmA (CS30) | F: AGTCAGCTCTTGCAGCCAGT | 0.2 | Mn | 219 | von Mentzer et al.[19] |
| R: CCTTGGTACCATTGCTGGTT |
CFs: Colonization Factors Mn: Monoplex PCR Mt: Multiplex-PCR. The numbers associated with each Mt correspond to the primers associated with each multiplex reaction.