Fig 4. Memory phenotype and cytokine production by Pk-antigen specific T cells elicited by RhCMV.

(A) Boxplots comparing the memory differentiation of the RhCMV/PK4 or ΔRh186-9/PK4-elicited CD4⁺ and CD8⁺ memory T cells in peripheral blood responding to each Pk antigen with TNF-α and/or IFN-γ production after the 2^nd boost (day 259). Memory differentiation state was based on CD28 vs. CCR7 expression, delineating central memory (T_CM), transitional effector memory (T_TREM), and effector memory (T_EM), as designated. (B) Boxplots comparing the frequency of vaccine-elicited CD4⁺ and CD8⁺ memory T cells in peripheral blood responding to each of the Pk antigens with production of TNF-α, IFN-γ, IL-2 or MIP-1β, alone and in all combinations.