Table 2. Sensitivity and specificity of commercial cELISA (ccELISA) and three versions of in-house cELISA (hcELISA) development for serological diagnosis of cattle infected with Anaplasma marginale or vaccinated with A. centrale.
| Antigen (cut-off point) | hcELISA | ccELISA | ||
|---|---|---|---|---|
| tMSP5m (%I > 30) |
tMSP5c (%I > 29) |
tMSP5cm (%I > 29) |
MBP-MSP5 (%I > 28) |
|
| Specificity (%) | 99.5 | 99.5 | 99.5 | 96.3 |
| Sensitivity (%) | 95.5 | 97.2 | 96.2 | 95.5 |
| Sensitivity (%) | 97.3 | 95.6 | 95.6 | 95.1 |
| Sensitivity (%) | 93.1 | 99.4 | 97.1 | 96.0 |
%I: percent inhibition.