KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-RBF (IF) | Du et al., 1996 | DX2 |
| mouse anti-RBF (WB) | Du et al., 1996 | DX3 |
| mouse anti-RBF (ChIP) | Du et al., 1996 | DX3:DX5 |
| rabbit anti-DP | Dimova et al., 2003 | 212 |
| rabbit anti-pH3 | Millipore | 06-570 |
| mouse anti-β-PS-integrin | DSHB | CF.6G11; RRID:AB_528310 |
| mouse anti-futsch | DSHB | 22c10; RRID:AB_528403 |
| mouse anti-β-tubulin | DSHB | E7; RRID:AB_528499 |
| mouse anti-β-Gal | DSHB | 40-1a; RRID:AB_528100 |
| rabbit anti-Mef2 | Lilly et al., 1995 | N/A |
| Critical Commercial Assays | ||
| SensiFast SYBR No-ROX Mix | Bioline | BIO-98020 |
| SensiFAST cDNA Synthesis Kit | Bioline | BIO-65053 |
| Deposited Data | ||
| Rbf and Dp ChIP-seq in muscles | This paper | GEO: GSE102043 |
| RNA-seq of muscles and notum in wild type and Me2 > Rbf-RNAi | This paper | GEO: GSE102104 |
| Drosophila EnsEMBL Biomart version 77, BDGP5.77 | EnsEMBL (Hubbard et al., 2007) | N/A |
| Database of known motifs Eurakyote DNA (Vertebrates in vivo and in silico) and FLY DNA | Tomtom | http://meme-suite.org/tools/tomtom |
| Transfac database, insect metrix (professional version release 2009.4) | Transfac | N/A |
| Experimental Models: Cell Lines | ||
| Drosophila melanogaster cell line: ML-Dmd8 | DGRC | Flybase: FBtc0000092; RRID:CVCL_Z754 |
| Experimental Models: Organisms/Strains | ||
| Act88F-GAL4 | Richard. M. Cripps | Bryantsev et al., 2012 |
| P{twi- bgal} | Richard. M. Cripps | N/A |
| vgAME-lacZ | Alexis Lalouette | N/A |
| P{GAL4-Mef2.R}3 | Bloomington Drosophila Stock Center | Flybase: FBst0027390 |
| UAS-Rbf-RNAi | Vienna Drosophila Resource Center | Flybase: FBst0450139 |
| UAS-Dp-RNAi | Vienna Drosophila Resource Center | Flybase: FBst0450633 |
| P{VALIUM20-mCherry}attP2 | Bloomington Drosophila Stock Center | Flybase: FBti0143385 |
| P{tubP-GAL80[ts]}20 | Bloomington Drosophila Stock Center | Flybase: FBti0027796 |
| UAS-usp-RNAiGD1554 | Vienna Drosophila Resource Center | Flybase: FBst0452461 |
| UAS-usp-RNAiJF02546 | Bloomington Drosophila Stock Center | Flybase: FBst0027258 |
| UAS-ct-RNAiGD1237 | Vienna Drosophila Resource Center | Flybase: FBst0470085 |
| UAS-Stat92E-RNAiHMS00035 | Bloomington Drosophila Stock Center | Flybase: FBst0033637 |
| UAS-Stat92E-RNAiJF01265 | Bloomington Drosophila Stock Center | Flybase: FBst0031317 |
| P{UAS-Dcr-2.D}1, w1118; P{GAL4-Mef2.R}R1 | Bloomington Drosophila Stock Center | Flybase: FBst0025756 |
| Oligonucleotides | ||
| Primer sequence | This paper | Table S7 |
| Software and Algorithms | ||
| TopHat version 2.1.1 | Trapnell et al., 2009 | N/A |
| DESeq2 version 1.14.1 with R version 3.3.2 (2016-10-31) | Anders and Huber, 2010 | N/A |
| BOWTIE version 2.3.2 | Langmead et al., 2009 | N/A |
| MACS version 1.4.2 | Zhang et al., 2008 | N/A |
| CEAS | Shin et al., 2009 | http://liulab.dfci.harvard.edu/CEAS/ |
| Integrative Genomics Viewer (IGV_2.3.79) | Robinson et al., 2011 | http://software.broadinstitute.org/software/igv/ |
| MEME | Bailey and Elkan, 1994 | http://meme-suite.org/tools/meme |
| Cytoscape v3.3.0 | Shannon et al., 2003 | https://cytoscape.org |
| STORM | Schones et al., 2007 | N/A |
| ImageJ 1.48v | NIH, USA | https://imagej.nih.gov/ij/download.html |