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. 2018 Jul 18;123(1):79–93. doi: 10.1093/aob/mcy141

Fig. 5.

Fig. 5.

Effect of treatment with methyl viologen (MV) and detection of superoxide ion in dcl1a-Ac plants. (A) Leaves of 3-week-old dcl1a-Ac (Osdcl1a-Ac#1) and wild-type (azygous) [WT(Az)] plants were treated with MV (10 μm). Right panels: quantification of chlorophylls (Chla+b) and carotenoids in mock-inoculated and MV-treated wild-type and dcl1a-Ac plants at 72 h after treatment. Data shown correspond to wild-type and dcl1a-Ac Plants. Scale bars = 250 μm. Data are the mean ± s.d. (*P ≤ 0.05; **P ≤ 0.01 by ANOVA). (B) Detection of superoxide ion radicals (O2·–) by nitroblue tetrazolium (NBT) staining. As a control, leaves were treated with H2O2 for 6 h. Three biological replicates with three technical replicates each were performed. Statistically significant differences were determined by one-way ANOVA.