. Author manuscript; available in PMC: 2020 Feb 1.

Published in final edited form as: J Immunol. 2018 Dec 31;202(3):1003–1015. doi: 10.4049/jimmunol.1800973

Table 3:

Masking strategy for FLICA aggregates. Mask name denotes the name given to individual mask. Function defines the type of mask used. Setting defines the parameters set for individual masks to achieve FLICA spot mask. FLICA-V mask identified all the FLICA aggregates in a cell. Spot count on FLICA-V was used to define diffused FLICA staining (spot count zero).

Mask name	Function	Setting
FLICA-I	Spot	Spot (M05, Ch05-FLICA, Bright, 5, 5, 2)
FLICA-II	Intensity	Intensity (FLICA-I, Ch05-FLICA, 150–4095)
FLICA Spot-III	Erode	Erode (FLICA-II, 1)
FLICA-IV	Dilate	Dilate (FLICA-III, 3)
FLICA-V	Range	Range (FLICA-IV, 20–1000, 0.6–1)