. Author manuscript; available in PMC: 2020 Feb 1.

Published in final edited form as: Ann Biomed Eng. 2018 Nov 28;47(2):366–380. doi: 10.1007/s10439-018-02171-3

Table 5:

Summary of the effects of endothelial cell attachment to collagen on hemostatic regulator molecule expression.

Substrate Application	Cell type	Molecules tested	Results
Collagen-derived peptide coat	Human umbilical vein endothelial cell; human aortic endothelial cell	PGI2, vWF; PECAM-1, VE-Cadherin, NOS3, TM, E-selectin;	Increase in NOS3 compared to TCPS; No significant change in PGI2, vWF; PECAM-1, VE-Cadherin, TM, E-selectin⁴⁹
Crosslinked collagen coat	Human umbilical vein endothelial cell; human saphenous vein	PGI2, vWF, tPA, PAI-1	Decrease in PGI2 and vWF secretion; No difference in basal levels of PGI2, tPA increase compared to bare^{53, 165}
ePTFE with collagen-1 coat	Human saphenous vein; human umbilical vein	tPA, PGI2, PAI-1	Decrease in PGI2 and tPA; no difference in tPA secretion; PAI increased; Different levels of PGI2, PAI-1 and tPA between unmodified versus modified PTFE^{53, 96, 175}
Collagen derived peptide hydrogel (PEG-Scl2)	Human aortic endothelial cell	PECAM-1, VE-Cadherin, NOS3, TM, E-selectin	Decrease in NOS3 and TM on PEG-Scl2 and E-selectin increased compared to collagen; no significant change in PECAM-1¹¹²