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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Neurooncol. 2018 Nov 20;141(2):289–301. doi: 10.1007/s11060-018-03047-1

FIG. 2.

FIG. 2.

Generation of iPSCs from primary LGG cells. (A, C) Bright field images of primary BT01 and BT03 cells. (B, D) Images of representative iPSC colonies derived from BT01 and BT03 cells. (E) LGG-iPSCs express alkaline phosphatase. (F-H) Immunostaining demonstrated that LGG-iPSCs express pluripotency-associated transcription factors (F) OCT4, (G) NANOG, and (H) SOX2. (I) Flow cytometry analysis confirmed LGG-iPSCs expressing the pluripotency-specific surface marker Tra-1–60. (J-L) Teratoma formation assay demonstrated LGG-iPSCs can differentiated into (J) ectoderm (neuroepithelium), (K) mesoderm (cartilage), and (L) endoderm (glandular structure). Scale bars, 100 μm. (M) Representative sequencing results of the BT01- and BT03-iPSC clones. More than 15 independent clones of each LGG samples were sequenced. None carried the IDH1 mutations.