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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Pain. 2019 Feb;160(2):334–344. doi: 10.1097/j.pain.0000000000001414

Figure 3. DA receptors D1 and D2 are expressed in different types of neurons in the Sp5C.

Figure 3

Immunofluorescence double staining was carried out to show co-localization of Cre with markers for different types of neurons in D1-Cre and D2-Cre mice. (A and B) In the Sp5C of D1-Cre mice, Cre-positive D1 neurons co-labeled with glutaminase (A) were more than those co-labeled with GABA (B). (C and D) In the Sp5C of D2-Cre mice, Cre-positive D2 neurons were primarily co-labeled with GABA (C) and only a few of those were co-labeled with glutaminase (D). In A–D, the upper panels display the expression of Cre and/or glutaminase/GABA in the Sp5C of D1-Cre mice (A and B) or D2-Cre mice (C and D) at low magnification. And the lower panels display the expression of Cre and/or glutaminase/GABA in the respective boxed areas of the upper panel at high magnification. The representative staining was repeated three times to confirm our results.