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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Feb;39(2):156–169. doi: 10.1161/ATVBAHA.118.312206

Figure 4. Mice with a single Akt isoform in hematopoietic cells have low levels of white blood cells, B-cells and monocytes but increased numbers of neutrophils and augmented monocyte apoptosis.

Figure 4.

(A-F); Blood cells were collected from retro-orbital sinus of mice reconstituted with WT, Akt1only or Akt3only FLC (n=4/group). Cells were incubated with antibodies to CD19, CD11b, Ly-6G, Ly-6C and CCR2, and then analyzed by multicolor flow cytometry. Graphs represent data (mean ± SEM, *p<0.05 compared to WT cells by One Way Analysis of Variance (Tukey test). The experiment was repeated three times.

(G-I) Total number of apoptotic cells (G), apoptotic neutrophils (H) and monocytes (I) in blood of mice transplanted with WT, Akt1only and Akt3only FLC. Cells were isolated from mice (n=3/group) and incubated with antibodies to CD11b, Ly-6G and then treated with Annexin V Alexa Flour 647 to detect apoptotic cells. The experiment was repeated two times. Graphs represent data (mean ± SEM), *p<0.05 compared to WT cells by One Way ANOVA.