Table I:
Primers for Amplification of Isolated Antibodies
| Heavy Chain Primers | Sequence |
|---|---|
| NB-VH1/5/7 | CAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTG |
| NB-VH2 | CAGATCACCTTGAAGGAGTCTGGTCCTACGCTGGTGAAACCCAC |
| NB-VH3 | GAGGTGCAGCTGGTGGAGTCTGGGGGAG |
| mutNB-VH4/6 | CAGGTGCAGCTGCAGGAGTCAGGCCCAGGACTG |
| CLONE-HeavyREV | CCAAGCTGCTGGAGGGCACGGTCACCACGC |
| CLONE-IgAREV | GCACTGTGTGGCCGGCAGGGTCAGCTGG |
| Light Chain Primers | |
| modNB-VK1 | GACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATC |
| modNB-VK2 | GATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCC |
| modNB-VK3 | GAAATTGTGTTGACGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAGG |
| modNB-VK4 | GACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTG |
| modNB-VK5 | GAAATTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCC |
| mod-VL1/2 | CTCATCACTCACTGTGCAGGGTCCTGGGCC |
| mod-VL3r | CTCGGCGTCCTTGCTTACTGCACAGGATC |
| mod-VL3p | CTCCCCCTCCTCACTCTCTGCACAGTCTC |
| NB-LightREV | GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTG |
| LambdaRev | CCTTCCAGGCCACTGTCACGGCTCC |
| Lambda Primers | |
| HuIgLVL5-A* | GGG AAT TCA TGR CCT GSW CYC CTC TCY TYC TSW YC |
| HuIgLVL3–1* | CCC AAG CTT GAA GCT CCT CAG AGG AGG G |