Figure 10: MT treatment inhibits APAP-induced RIP3 kinase activation and RIP3 kinase deficiency promotes caspase 3 activity.
Mice were treated with 300 mg/kg APAP, followed by 20 mg/kg MT or saline 1.5 h later. (A) Western blot for RIP3 kinase protein levels 24h after APAP and (B) densitometric quantitation of western blot. (C) Western blot showing caspase cleavage and (D) caspase 3 activity in liver homogenates. Bars represent means ± SEM for n = 3 mice per group. *p<0.05 vs. APAP (B) and *p<0.05 vs. Ctrl. (D).