Figure 2.

The Naive Subpopulation Is Transcriptionally Distinct from the Other Naive and Primed Cells

(A) Heatmap of the top 50 genes with the strongest differential expression between the naive and intermediate cells (top) or between the intermediate and primed cells (bottom). The box for each cell (column) and gene (row) is colored according to the log₂-fold change from the average expression for each gene.

(B) Log₂ expression profiles of selected marker genes across cells in the naive, intermediate, and primed populations. Each point represents a cell in the corresponding population.

(C) Normalized protein expression of DPPA5 against ABCG2 in naive and primed hESCs. Protein expression was determined using immunofluorescence staining of cytospin-fixed cells.

(D) Representative immunofluorescence images of naive and primed hESCs using DPPA5 and ABCG2 antibodies. The scale bar represents 20 μm.

See also Figure S2 and Table S2.