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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: Mol Neurobiol. 2018 Apr 27;56(1):378–393. doi: 10.1007/s12035-018-1068-y

Figure 7: Despite stable expression of SpCas9 and GMF sgRNAs, GMF editing is moderate:

Figure 7:

Flow cytometric analysis of GMF gene editing in BV2 cells revealed that as compared to the normal GMF expression in BV2 cells, GMF gene editing leads to significant reduction in GMF expression especially due to GMF sgRNA1 and sgRNA2. Surprisingly there was no significant change in GMF expression due to GMF sgRNA3-mediated GMF gene editing. The changes in GMF expression are indicated in terms of GMF positive and GMF negative cell populations.