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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: Mol Neurobiol. 2018 Apr 27;56(1):378–393. doi: 10.1007/s12035-018-1068-y

Figure 8: GMF gene editing causes suppression of MAPK pathway activation:

Figure 8:

Wild type BV2 cells, and GMF gene edited BV2 cells were treated with 1.0μg/ml LPS for 30 minutes and analyzed for the expression of pp38MAPK and p38 MAPK. The representative western blot data (n=3) indicates that wild type BV2 cells display enhanced pp38 MAPK and p38 MAPK expression in the presence of LPS treatment (lanes 1 and 2). However, GMF sgRNA2-edited BV2 cells exhibit significant reduction in both pp38 MAPK and p38 MAPK expression with and without LPS treatment (lanes 5 and 6). Surprisingly, pp38 MAPK and p38 MAPK expression patterns are very similar between wild type as well as GMF sgRNA1 and sgRNA3-edited BV2 cells as depicted in lanes 3, 4 and 7, 8 respectively.