Table 2.
The comparison of structural signatures among structural groups of Ni-CODHs.
| Structural group | D-cluster | C-cluster |
Acid-base |
E- & F-clusters | Novel structural motifs | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| His | Cys1 | Cys2 | Cys3 | Cys4 | Cys5 | His | Lys | ||||
| A-1 | type I | H | C | C | C | C | C | H | K | + | |
| A-2 | – | H | C | C | C | C | C | H | K | + | |
| A-3 | – | H | C | C | C | C | C | H | K | – | |
| A-3 | – | H | C | C | C | C | C | H | K | – | |
| A-4 | – | D | P | C | C | C | C | Q | R | + | |
| A-5 | – | H | C | C | C | C | C | H | K | + | Two Cys × 4 motifs between E- and F-clusters |
| A-6 | – | N | S | C | C | C | C | H | L | +b | |
| B-1 | type II | H | C | D/E | C | C | C | H | K | – | His-rich region at the N-terminus |
| B-2 | type II | H | C | D | C | C | C | H | K | – | |
| B-3 | type II | H | C | D | C | C | C | H | K | – | His-rich region at the C-terminus |
| B-4 | type II | H | C | C | C | C | C | H | K | – | |
| C-1 | type II | H | C | C | C | C | C | Y | Q | – | A Cys × 2 to 4 motif at the N-terminus |
| C-2 | type II | H | C | C | C | C | C | Y | Q | – | |
| C-3 | –a | H | C | C | C | C | C | Y | Q | – | |
| C-4 | type II | H | C | C | C | C | C | Y | Q | – | A Cys × 4 motif between the Tyr and His residues |
| D-1 | type III | H | E | C | C | C | C | Y | K | – | |
| D-2 | type III | H | C | C | C | C | C | N | K | – | |
| D-3 | type II | H | C | C | C | C | C | Y | K | – | A Cys × 3 motif at the N-terminus |
| D-4 | type II | H | C | C | C | C | C | Y | K | – | |
| E-1 | type II | H | C | C | C | C | C | H | K | – | |
| E-2 | type III | H | C | C | C | C | C | H | K | – | |
| E-3 | type II | H | C | C | C | C | C | H | K | – | A Cys × 2 to 3 motif at the N-terminus |
| F-1 | type II | H | C | C | C | C | C | H | K | – | |
| F-2 | type II | H | C | C | C | C | C | H | K | – | A Cys × 2 to 3 motif at the N-terminus |
| G-1 | type III | H | T | C | C | C | C | Y | K | – | |
aOne Cys residue was substituted to other amino acids.bOne Cys residue in the F-cluster was substituted to other amino acids.