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. 2019 Jan 17;11:491. doi: 10.3389/fnmol.2018.00491

FIGURE 2.

FIGURE 2

En1Cre driven deletion of Ezh2 affects the amount of TH+ cells at E14.5. Protein expression of TH was evaluated at E12.5 (A) and E14.5 (B). (A) Quantification of the amount of TH positive cells in sagittal midbrain sections at E12.5 demonstrated no significant changes in the number of TH+ cells between the En1Cre/+;Ezh2 +/+ (n = 3, black bar) and En1Cre/+; Ezh2 L/L (n = 3, gray bar) embryos. Wildtypes were set at 1. In addition TH+ neurons were normally distributed over the lateral and medial mdDA domain. Scale bars = 400 μM. (B) Quantification of the amount of TH+ in sagittal midbrain sections at E14.5. The total amount of TH+ neurons were reduced with ∼15% in the Ezh2 cKO embryos (n = 3, ∗P < 0.05, two-tailed). Subdivision into the lateral and medial population based on the location of the retroflex fasciculus demonstrates that in the lateral population ∼41% of the TH+ cells are lost in En1Cre/+; Ezh2 L/L embryos (4, white arrowhead) (n = 3, ∗∗P < 0.01, two-tailed), while an upward trend in the amount of TH+ cells in the medial domain is observed (2, arrow) (n = 3, P = 0.089, two-tailed). Wildtypes were set at 1. Scale bars = 400 μM and for 1–4 80 μM.