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. 2019 Jan 17;9:3188. doi: 10.3389/fimmu.2018.03188

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Copyright © 2019 De Pelsmaeker, Devriendt, De Regge and Favoreel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Porcine NK cells internalize fragments of killed PRV-infected target cells. (A) Histograms show the CFSE signal of IL-2-primed NK cells that were incubated for the indicated times with PRV WT-infected SK cells (NK:target ratio 25:1) that had been labeled with CFSE (red open histogram), CFSE-labeled mock-infected SK cells (black open histogram) or not incubated with target cells (gray shaded histogram) of one representative pig (out of three). The amount of CFSE-positive cells (%) is indicated in the histograms. (B) Graph shows the amount of CFSE-positive IL-2-primed NK cells that were co-incubated for the indicated times with CFSE-labeled mock-infected SK cells or PRV wild type-infected SK cells (effector target ratio of 25:1). Dot plot shows the results of three individual blood donors and the mean values are connected with a line. P-value threshold for statistically significant differences was 0.05, p-values are indicated in the graphs.