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. 2019 Jan 23;9:376. doi: 10.1038/s41598-018-36700-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Infectious RML prion rods imaged by negative stain EM, cryo-EM and AFM. (a,b) Negative stain EM images using (a) uranyl acetate at pH 4 and (b) NanoW at pH 6.8. (c,d) Cryo-EM images. (e) 140 nm × 140 nm AFM scan revealing the presence of material in the central gap of the rod which appears to link the paired fibres. Main panel 3D image, inset, 2D image. (f) AFM surface topography (height) adhesion force mode showing that material in the central gap region of the rod interacts more strongly with the scanning tip (exerts a greater adhesive force) than the rod fibres (light to dark representing high to low adhesive force). Scale bars, 100 nm main panels a, b and d, 200 nm panel c, 10 nm main panels e and f and insets in panels a and e.