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. 2019 Jan 23;9:389. doi: 10.1038/s41598-018-37117-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rh-BDNF reduces fibrin fibers density in vitro. Purified fibrin/thrombin clots were formed with recombinant BDNF (rh-BDNF: 100–250 pg/ml) or with BSA (1 mg/ml; control: point 0). (a) Representative image of clots using Alexa Fluor 488–labeled fibrinogen (20X magnification), and (b) percentage of fibrin fibers versus control. Fibrin fibers were analyzed using Image J software. All samples were performed in triplicate. Data are expressed as mean ± SEM; n = 9 *p < 0.05 ***p < 0.005.