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. 2019 Jan 23;9:389. doi: 10.1038/s41598-018-37117-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Low circulating BDNF levels are associated with higher density of fibrin fibers and greater MCF in CHD patients. Plasma pools of two or three CHD patients or healthy subjects (Control) were obtained according to similar BDNF levels (±20 pg/ml): (a) BDNF levels, (b) fibrin fibers density, and (c) Maximum Clot firmness (MCF) have been analysed by ELISA kit, Alexa Fluor 488–labeled method (20X magnification) and thromboelastographic analyses, respectively. Correlation between BDNF concentrations and (d) MCF. All samples were performed in triplicate, and representative images are shown. Data are expressed as mean ± SEM. n = 12 and 9 pools of CHD and healthy subjects, respectively.