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. 2019 Jan 23;9:389. doi: 10.1038/s41598-018-37117-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rh-BDNF reduces fibrin clot profile of CHD patients in vitro. Recombinant BDNF (rh-BDNF; 5, 25, 135 and 270 pg/ml) or BSA (1 mg/ml: control) was added to plasma from CHD patients before induction of coagulation with thrombin, and fibrin fibers were (a) visualized with Alexa Fluor 488–labeled (20X magnification) and (b) quantified using Image J software. All samples were performed in triplicate and representative images are shown. Data are expressed as mean ± SEM; horizontal bars indicate variation of BDNF levels measured in plasma pools analyzed. n = 12 plasma sample from CHD patients with BDNF < 100 pg/ml. *p < 0.05, **p < 0.01 and ***p < 0.005.