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. 2019 Jan 23;9:341. doi: 10.1038/s41598-018-36588-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interferon and regulatory cytokine profile in lung and spleen. MFI of each gene was normalized to the average of HKGs – (Hprt 1 and Tubb 1), then fold changes were calculated against the saline control at each time point and the means were graphed. (Dotted line shows a fold change of one, which indicates no change compared to the control group and the vertical line and horizontal bar show the standard error of mean-SEM). Dunnett’s test following ANOVA testing was used to test for significant differences of gene expression between CpG-ODN group and the saline control group at each time point. Asterisks indicate groups that were significantly different from control group, *P < 0.05 and **P < 0.01.