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. 2019 Jan 23;9:341. doi: 10.1038/s41598-018-36588-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric analysis of lung cells 48 (a) and 72 hrs (b) post in-ovo injections. (Day 20 and 21 embryo). Macrophages were analyzed (left panels) by gating on monocyte/macrophages population based on forward and side scatter plot. Histogram panels on right indicate the level of CD86 and CD40 (costimulatory molecules found on APCs) expression on the APCs. (c) Bar diagram show the mean percentages of monocyte/macrophage APCs following in-ovo treatment in lung. vertical line and horizontal bar show the standard error of mean SEM, n = 3–4. Two way ANOVA following Bonferroni post-test was done to compare means of APC percentages of CpG-ODN received groups with saline control. Asterisks indicate significant differeces between the groups, **P < 0.01.