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. 2019 Jan 23;9:326. doi: 10.1038/s41598-018-36841-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Suppression of profibrotic response in precision cut liver slices. (A) Analysis of procollagen accumulation in slices. Freshly prepared rat liver slices (day 0, slices 1–6), the slices cultured for 3 days without treatment (day 3, slices 1–6) and the slices treated with 1 μM of C9 from day 0 to day 3 (day 3 + C9, slices 1–6) were homogenized and type I collagen analyzed by western blot. Arrow indicates mature collagen and double arrow indicates nascent procollagen. Loading controls, actin and fibronectin. (B) Quantification of results. Intensity of bands representing procollagen was measured by densitometry from the slices shown in A and normalized to the actin band and plotted as arbitrary units (A.U.) P = 0.0184 by unpaired t test, error bars: +−SEM.