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. 2019 Jan 23;9:423. doi: 10.1038/s41598-018-36714-4

Table 1.

oligonucleotide sequences for real-time RT-PCR.

Oligonucleotide Name Sequence 5′-3′
DENV gene oligonucleotide sequences
Type 1–5′ NS5 5′- CAGGTCAAACGCAGCTATTG
Type 1–3′ NS5 5′- CCACTCCACTGAGTGAATTC
Type 2–5′ NS5 5′- TGTATGCCGATGACACCGCA
Type 2–3′ NS5 5′- TCTTTGCACACGGACCACCT
Type 3–5′ NS5 5′- TCAGAACTAACGCAGCCATG
Type 3–5′ NS5 5′- AGAGTTTTCACGCGAGAACC
Type 4–5′ NS5 5′- AGATCAAACGCAGCCATAGG
Type 4–5′ NS5 5′- CTTCCACTCCACTCCATGAA
Human gene oligonucleotide sequences
5′ GAPDH 5′-GTCTTCACCACCATGGAGAA
3′ GAPDH 5′-ATGGCATGGACTGTGGTCAT
5′ OAS1 5′- CAAGCTTAAGAGCCTCATCC
3′ OAS1 5′- TGGGCTGTGTTGAAATGTGT
5′ OAS2 5′- ACAGCTGAAAGCCTTTTGGA
3′ OAS2 5′- GCATTAAAGGCAGGA AGCAC
5′ OAS3 5′- CACTGACATCCCAGACGATG
3′ OAS3 5′- GATCAGGCTCTTCAGCTTGG
5′ PKR 5′- ATGATGGAAAGCGAACAAGG
3′ PKR 5′- GAGATGATGCCATCCCGTAG
5′ IFN-alpha 2 5′-GCA AGT CAA GCT GCT CTG TG
3′ IFN-alpha 2 5′-GAT GGT TTC AGC CTT TTG GA
5′ IFN-alpha 17 5′-AGG AGT TTG ATG GCA ACC AG
3′ IFN-alpha 17 5′-CAT CAG GGG AGT CTC TTC CA