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. 2019 Jan 23;9:426. doi: 10.1038/s41598-018-36909-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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High-fat diet promotes weight gain and altered metabolic parameters. (A) Body weight of CD or HFD mice were monitored over 16 weeks. (B) Ventral view of the mice showing the changes in the total abdominal length caused by the two diets after 16 weeks. (C–J) Effect of HFD on metabolic parameters. Measurement of serum resistin (C), leptin (D), insulin (E), adiponectin (F), total cholesterol (G), LDL (H), HDL (I) and triglyceride (J). (K) Immunostained section of the infrapatellar fat pad (IFP) of mice fed a control or HFD diet. Bar = 100 µm. Graphs represent mean ± SD (n = 7). *p < 0.05. CD, control diet-fed mice; HFD, high fat diet-fed mice.