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. 2019 Jan 23;9:362. doi: 10.1038/s41598-018-36613-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Re-hydration profile of 3D CH-based scaffolds previously de-hydrated at different percentages and left in water as a function of time. Scaffolds were gelled in (A) KOH (1.5M), (B) Na₂CO₃ (1.5M), (C) NH_3(g) (from 28% ammonia solution) and exposed to vacuum oven at 40 °C at different times to obtain the desired lost percentage of water, namely 30% (cross), 45% (solid triangle), 60% (solid square) or 85% (solid circle) of initial water weight.