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. 2019 Jan 23;4(1):e00689-18. doi: 10.1128/mSphere.00689-18

TABLE 1.

Effects of pilocarpine hydrochloride and acetylcholine on hemocyte cellularity and subtype characteristics in vitro and in vivo

Condition
and treatmenta 		Cellularity Hemocyte subsetb
Nodule
 C. albicansc
Pr Gr Pl Co Sp Ad Oe Size Melanization
In vitro
    CTR Low + + − − + + − − − −
    SC5314 High + ++ ++ ++ ++ + + Medium ++ +++
    ACh High + ++ +++ + − − − Medium − −
    PHCl Low + + − − + + − − − −
    SC5314/ACh High + +++ +++ ++ + + − Large − +
    SC5314/PHCl High + + +++ + + ++ + Small − +
In vivo
    CTR Low + + − − + + − − − −
    SC5314 High + +++ ++ ++ ++ + + Large +++ +++
    ACh Intermediate + ++ +++ + − − − Medium − −
    PHCl Low + + + − + + − − − −
    SC5314/ACh Intermediate + +++ +++ ++ + + + Large − +
    SC5314/PHCl Intermediate + ++ +++ ++ + + + Small − +
a

Galleria mellonella larvae were inoculated with PBS (control [CTR]), C. albicans SC5314, acetylcholine (ACh), pilocarpine hydrochloride (PHCl), C. albicans plus ACh (SC3514/ACh), and C. albicans plus PHCl (SC5314/PHCl).

b

Pr, prohemocytes; Gr, granulocytes; Pl, plasmatocytes; Co, coagulocytes; Sp, spherulocytes; Ad, adipocytes; Oe, oenocytes. Immune cell subtype quantification was scored as follows: −, absent/rare; +, 1 to 10%; ++, 11 to 30%; +++, 31 to 50%.

c

C. albicans presence was quantified as follows: −, absent; +, few cells; ++, multiple yeast agglomerate usually embedded in nodules; +++, abundant yeasts and/or hyphae with widespread diffusion in nodules.