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Pairwise alignment of MerA ATII-LCL and ATII-LCL-NH. The amino acids different in ATII-LCL compared with ATII-LCL-NH are in red. The NmerA domain (55) is overlined in green. The dimerization domain (61) is overlined in purple. The cysteine pairs 11/14 and 558/559, which are responsible for Hg2+ binding, and the cysteine pair 136/141 involved in the catalytic site are highlighted in yellow. Positions of the amino acids involved in the mutations performed in this work are in black boxes.
