TABLE 3.
Kinetics data of ATII-LCL-NH, 2D, 4D, and 4DB1B2 mutantsa
| MerA isoform | Sp act (μmol/min/mg) | Km (μM) | Vmax (μmol/min/mg) | kcat (s−1) | kcat/Km |
|---|---|---|---|---|---|
| ATII-LCL-NH | 10.96 ± 0.74 | 11.25 ± 0.71 | 14.21 ± 0.81 | 12.25 ± 1.2 | 1.1 |
| 2D | 10.95 ± 0.58 | 10.96 ± 0.82 | 14.05 ± 0.73 | 12 ± 1 | 1.1 |
| 4D | 11.1 ± 0.6 | 13.9 ± 1.35 | 15.09 ± 0.93 | 12.6 ± 1.4 | 0.9 |
| 4DB1B2 | 10.9 ± 0.68 | 12.8 ± 0.93 | 14.4 ± 0.78 | 12.4 ± 0.98 | 0.96 |
a
Data were determined by Lineweaver-Burk (double reciprocal plot) and GraphPad Prism software (GraphPad, La Jolla, CA, USA).