TABLE 2.

List of CAZymes significantly upregulated during monoculture and coculture growth of B. hungatei MB2003 on xylan^a

Locus tag	Name	Annotation	CAZy	FDR	log2 fold change
MB2003 monoculture growth
bhn_I2653	cel9A	Endo-1,4-β-glucanase	GH9/CelD	1.42E−77	5.36
bhn_I1780	xyn8A	Reducing-end xylose-releasing exo-oligoxylanase	GH8	7.38E−98	4.2
bhn_I0856	est4C	Polysaccharide deacetylase	CE4	1.57E−05	3.12
bhn_I0203	gh2B	Glycosyl hydrolase family 2	GH2	4.01E−16	2.7
bhn_III78	est1A	Feruloyl esterase	CE1	1.17E−17	2.69
bhn_I2715	pgl28B	Polygalacturonase	PL3, GH28	1.03E−02	2.54
bhn_I2139	xsa43D	Xylosidase/arabinofuranosidase	GH43	9.88E−03	2.49
bhn_III76	lyc25B	Lysozyme	GH25, 2× SH3	1.73E−18	2.17
MB2003 coculture growth
bhn_I0681	agn53A	Arabinogalactan endo-1,4-β-galactosidase	GH53	1.78E+10	3.7
bhn_I2221	gh31C	Glycoside hydrolase family 31	GH31	1.73E+40	2.98
bhn_I1841	bga35B	β-Galactosidase	GH35	9.70E+11	2.66
bhn_I1532	arf51C	α-l-Arabinofuranosidase	GH51/CBM4	4.33E+22	2.25
bhn_I1407	est4D	Polysaccharide deacetylase	CE4	5.31E−03	2.23
bhn_I0167	cel5A	Endo-1,4-β-glucanase/xylanase	GH5	4.19E−03	2

^aGenes were considered significantly expressed if all three thresholds were met, i.e., FDR calculated using Benjamini-Hochberg Q values of <0.05, Kruskal-Wallis test P values of <0.05, and log₂ ≥2-fold difference in expression.