TABLE 3.
List of CAZymes significantly upregulated during monoculture and coculture growth of B. hungatei MB2003 on pectina
| Locus tag | Name | Annotation | CAZy | FDR | log2 fold change |
|---|---|---|---|---|---|
| MB2003 monoculture growth | |||||
| bhn_III64 | bgl3A | β-Glucosidase | GH3 | 5.66E+53 | 6.13 |
| bhn_III76 | Lysozyme | GH25 | 2.98E+19 | 6.04 | |
| bhn_I2226 | cbp94A | Cellobiose phosphorylase | GT36 | 1.10E+86 | 5.83 |
| bhn_I0187 | gh115A | α-Glucuronidase | GH115 | 4.39E+53 | 4.94 |
| bhn_I1972 | aga36C | α-Galactosidase | GH27 | 4.38E+28 | 4.54 |
| bhn_I0084 | gh27A | α-Galactosidase | GH27 | 8.45E+34 | 4.5 |
| bhn_I0681 | agn53A | Arabinogalactan endo-1,4-β-galactosidase | GH53 | 1.71E+22 | 4.36 |
| bhn_I0307 | est1B | Feruloyl esterase | CE1 | 2.56E+23 | 4.25 |
| bhn_I0092 | gh105B | Unsaturated rhamnogalacturonyl hydrolase | GH88/GH105 | 1.26E+16 | 4.15 |
| bhn_I1604 | gh31A | Glycoside hydrolase family 31 | GH31 | 5.45E+35 | 4.14 |
| bhn_I2738 | amy13A | α-Amylase | GH13 | 7.67E+18 | 4.11 |
| bhn_I2139 | xsa43D | Xylosidase/arabinofuranosidase | GH43 | 9.78E+18 | 4.09 |
| bhn_I1308 | aga27A | α-Galactosidase | GH27 | 1.12E+16 | 4.02 |
| bhn_I0286 | gh31B | α-Glucosidase | GH31 | 4.66E+14 | 3.91 |
| bhn_I0192 | xyl3A | β-Xylosidase | GH3 | 4.58E+12 | 3.85 |
| bhn_I0183 | Acetyl-xylan esterase | GH2/DUF303 | 9.96E+15 | 3.77 | |
| bhn_I2771 | gh30A | Glycoside hydrolase family 30 | GH30 | 5.91E+13 | 3.68 |
| bhn_I0654 | aga36A | α-Galactosidase | GH27 | 5.20E+10 | 3.56 |
| bhn_I0680 | amy13D | α-Amylase | GH13 | 1.39E+06 | 3.51 |
| bhn_I2238 | pbp1A | Penicillin-binding protein 1A family | GT51 | 8.06E+09 | 3.51 |
| bhn_I0169 | xsa43A | Xylosidase/arabinofuranosidase | GH43/CBM6 | 1.89E+09 | 3.41 |
| bhn_I0840 | bga2C | β-Galactosidase | GH2 | 4.62E+06 | 3.3 |
| bhn_I1336 | glgX2 | Glycogen debranching enzyme | GH13 | 3.69E+06 | 3.27 |
| bhn_I2065 | xsa43C | Xylosidase/arabinofuranosidase | GH43 | 1.33E+06 | 3.26 |
| bhn_I1873 | Carbohydrate-binding protein | CBM2/6xCBM6 | 4.24E+09 | 3.24 | |
| bhn_I0185 | agu67A | α-d-Glucuronidase | GH67 | 2.48E+08 | 3.23 |
| bhn_I1407 | est4D | Polysaccharide deacetylase | CE4 | 9.58E−05 | 3.21 |
| bhn_I1967 | Mannose-6-phosphate isomerase | GH1 | 2.31E+06 | 3.04 | |
| bhn_I2434 | est4B | Polysaccharide deacetylase | CE4 | 8.83E−03 | 2.76 |
| bhn_I0579 | xyn10B | Endo-1,4-β-xylanase | GH10 | 6.33E−04 | 2.7 |
| bhn_I2332 | pgl28B | Polygalacturonase | GH28 | 1.31E−02 | 2.69 |
| bhn_I1961 | cel5C | Endo-1,4-β-glucanase | GH5/CBM2 | 1.35E−02 | 2.68 |
| bhn_I1060 | amy13B | α-Amylase | GH13 | 2.71E−02 | 2.56 |
| bhn_I2290 | gh31C | Glycoside hydrolase family 31 | GH31 | 4.13E−02 | 2.44 |
| bhn_I0644 | amy13B | α-Amylase | GH13 | 3.49E−03 | 2.42 |
| bhn_I2551 | bga2A | β-Galactosidase | GH2 | 4.09E−04 | 2.41 |
| bhn_I0194 | lyc25C | Lysozyme | GH25 | 2.32E−02 | 2.13 |
| bhn_I1969 | Glycoside hydrolase GH130 family | GH130/GH43 | 2.15E−02 | 2.11 | |
| bhn_I2221 | gh31C | Glycoside hydrolase family 31 | GH31 | 6.89E−03 | 2.07 |
| bhn_III72 | est4A | Polysaccharide deacetylase | CE4 | 0.00E+00 | N/A |
| MB2003 coculture growth | |||||
| bhn_I1532 | arf51C | α-l-Arabinofuranosidase | GH51/CBM4 | 0.00E+00 | 5.05 |
| bhn_I0847 | cel9B | Cellodextrinase | GH9/CelD | 1.74E−31 | 3.35 |
a
Genes were considered significantly expressed if all three thresholds were met, i.e., FDR calculated using Benjamini-Hochberg Q values of <0.05, Kruskal-Wallis test P values of <0.05, and log2 ≥2-fold difference in expression.