TABLE 4.
TaqMan primers/probes used to enumerate culture growth
| Primer/probe | Specificity | Gene annotation | Locus tag | Amplicon size (bp) | Sequence |
|---|---|---|---|---|---|
| buk_Forward | B316T and MB2003 | Butyrate kinase (buk) | bpr_I2323 | 90 | 5′-ACCTTGGAACCAACGATATGAG-3′ |
| buk_Reverse | 5′-GGTAAATGAAAGCGTCACGAAC-3′ | ||||
| buk_Probe | 5′-TGTGTGATGAGGGCAACGAGAAGG-3′ | ||||
| CBD_Forward | B316T | Cell-wall-binding-domain-containing protein | bpr_I0264 | 86 | 5′-CGCTACAGAGGGAACTGAAATAG-3′ |
| CBD_Reverse | 5′-CCTCAAGGCTCTCATCAACTAC-3′ | ||||
| CBD_Probe | 5′-CTGTGGAAGCATCAAGCGCAGC-3′ | ||||
| Arf51C_Forward | MB2003 | α-l-Arabinofuranosidase (arf51C) | bhn_I1532 | 88 | 5′-GCAAAGGTATGTTTGGACTGTT-3′ |
| Arf51C_Reverse | 5′-AAACTACGGTTCTCAAGCATTTC-3′ | ||||
| Arf51C_Probe | 5′-TGCATGAAGACCACCGTCAAGACC-3′ |