FIG 3.
Biofilms formed in the presence of iron can be dispersed by NO. (A) P. aeruginosa biofilms were grown in multiwell plate batch cultures for 6 h and pretreated with 100 μM FeCl3 for 30 min. Biofilms were then treated with 100 μM SP-NO for 15, 30, or 60 min before CV staining. (B) Six-hour biofilms were pretreated with 100 μM FeSO4 for 30 min. Biofilms were then treated with 100 μM SP-NO for 5 to 60 min before CV staining. Error bars indicate standard deviations (n = 4). ***, P ≤ 0.001 versus untreated control samples.