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Iron-limited biofilms become more sensitive to NO. Biofilms grown in multiwell plate batch cultures for 6 h were treated with 50 μM SP-NO, 200 μM Bipy, or the combination of both for 15, 30, and 60 min. Biofilm biomass was quantified by CV staining. Error bars indicate standard deviations (n = 6). *, P ≤ 0.05; ***, P ≤ 0.001 versus untreated control samples or between different samples.
