TABLE 1.
Primer sets designed for pahE genes and the primer sets chosen for pahAc genes
| Primer | Sequencea | Length (nt) | Degeneracy | Length of PCR products (bp) | Tm (°C)b | Reference |
|---|---|---|---|---|---|---|
| pahE1F | TGCGGCGGGTGTNAAYGGNAT | 21 | 32 | 377 | 57 | This study |
| pahE1R | CCTGAGGAATCTCGGACATYTSTGCCCARAA | 31 | 8 | |||
| pahE2F | AGCATGGGAACKYTKGGNGA | 20 | 32 | 366 | 52 | This study |
| pahE2R | TTTGGCGGTVACVACYTG | 18 | 18 | |||
| pahE3F | GACGGCGTSGACGGVATCAT | 20 | 6 | 323 | 54 | This study |
| pahE3R | TCAGGGTTGTCRTARAKSA | 19 | 16 | |||
| pahE4F | TGGTGCGYGAYGGBGYCGA | 19 | 24 | 324 | 54 | This study |
| pahE4R | GGCGTGCGGGTTSTSRTARAYCA | 23 | 32 | |||
| PAH-RHDα-396F | ATTGCGCTTAYCAYGGBTGG | 20 | 12 | 320 | 57 | 16 |
| PAH-RHDα-696R | ATAGGTGTCTCCAACRAARTT | 21 | 4 |
a
The pahE primers consisted of a 5′ consensus clamp region and a 3′ degenerate core region (italicized in the primer sequences).
b
Tm, melting temperature.