TABLE 2.
Comparisons of the amplification of pahAc and pahE from reference strains by pahAc and pahE primer sets, respectively
| Reference strain | Substrate(s)a | Amplification results with primer sets forb: |
|
|---|---|---|---|
| pahAc | pahE | ||
| PAH-degrading bacteriac | |||
| Pseudomonas stutzeri 1-5 | Phn/Pyr | + | + |
| Delftia sp. strain Cs4-1 | Phn | + | + |
| Marinomonas profundimaris D104 | Nah/Ant/Phn/Pyr | + | + |
| Sphingomonas sp. strain 1-1 | Phn | + | + |
| Novosphingobium pentaromativorans US6-1 | Nah/Ant/Phn/Pyr/BaP | + | + |
| Rhodococcus sp. strain B4 | Nah | + | + |
| Mycobacterium vanbaalenii PYR-1 | Nah/Ant/Phn/Pyr/Fla | + | + |
| Thioclava dalianensis DLFJ1-1 | New potential degrader | + | + |
| Other aromatic hydrocarbon-degrading bacteriad | |||
| Pseudomonas stutzeri ZWLR2-1 | 2-CNB | + | − |
| Comamonas sp. strain CNB-1 | 3-CNB | + | − |
| Other strains | |||
| E. coli DH5α | NO | − | − |
| Bacillus subtilis 168 | NO | − | − |
a
Phn, phenanthrene; Pyr, pyrene; Nah, naphthalene; Ant, anthracene; BaP, benzopyrene; Fla, fluoranthene; 2-CNB, 2-chloronitrobenzene; 3-CNB, 3-chloronitrobenzene; NO, not capable of degrading aromatic hydrocarbons.
b
+, correct sequence was amplified. −, no amplicon.
c
Can mineralize PAHs or grow on PAHs as the only carbon source.
d
Cannot mineralize PAHs or grow on PAHs as the only carbon source.