FIG 1.

Intestinal microbiome composition varies over a feeding cycle. (A) Principal coordinate analysis showing significant microbiome clustering by time of sampling (ANOVA, P < 0.05). Distance was based on Bray-Curtis similarities with all samples rarefied to 20,562 sequences. Samples within the dashed circle were primarily from the unfed-state time points while samples within the solid circle were from the fed-state time points. (B) Relative abundances of the two most common ESVs (exact sequence variants) vary over a feeding cycle (n = 17 at 1100 and 2000 h; n = 18 at 1230, 1400, and 1600 h). ANCOM identified the Photobacterium ESV but not the Clostridium ESV as varying significantly over the sampling period (P < 0.05).