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Time course of the E1 consumption as well as the production of 4-norestrogenic acid and HIP in strains KC8 (A) and SLCC (B). The resting cells were aerobically incubated with E1 (1 mM). The [2,4,16,16-D4]E1 and unlabeled E1 were mixed at a 1:1 molar ratio. Quantification of the estrogen metabolites was based on pesudomolecular ion counts corresponding to unlabeled compounds using UPLC-ESI-HRMS. Data shown are the means ± SD from three experimental measurements.
