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Extracted ion chromatograms (EIC) and MS spectra of two authentic standards, CoASH (A) and benzoyl-CoA (B), and the E1-derived 4-norestrogen-5(10)-en-3-oyl-CoA (metabolite 4) (C). In the resting cell assay of strain KC8, the substrate was composed of [2,4,16,16-D4]E1 and unlabeled E1 (mixed at a 1:1 molar ratio). The fragment ion (m/z 768.12) peak corresponding to the CoA moiety was observed in the MS spectra of benzoyl-CoA and 4-norestrogen-5(10)-en-3-oyl-CoA.
