The periostin and TSLP levels were significantly higher in eosinophilic than noneosinophilic polyp tissues. (A) Representative HE, IHC staining with periostin and TSLP, AB-PAS and Masson staining (200× magnification); (B,C,D,E,F) the representative western blot results of periostin protein (B), the mRNA expression of periostin (C) and TSLP (E), ELISA analysis of periostin (D) and TSLP (F) in eosinophilic and noneosinophilic polyp tissues. ***, P<0.001 as determined by Mann Whitney U test. HE, hematoxylin and eosin; IHC, immunohistochemistry; AB-PAS, Alcian new blue and periodic acid-Schiff; TSLP, thymic stromal lymphopoietin; NP, nasal polyp; EOS, eosinophils.