Figure 5.

Effects of S1 nuclease treatment on FFPE-associated somatic SNV noise. Libraries were prepared from fresh normal and tumor samples from the same patient, and matching FormaPure FFPE samples with (F+S1) and or without (F-S1) S1 nuclease treatment. For each of the three latter libraries, SNVs were identified relative to the library from the normal blood sample. Upset plots indicating data overlaps are shown. In (A) are data obtained using a QSS score cutoff ≥15 as a cut-off. In (B) are data generated using a QSS score cutoff ≥35.