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. 2018 Nov 29;47(2):843–854. doi: 10.1093/nar/gky1201

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Inactivation of the ACT domain of SpoT leads to (p)ppGpp accumulation, which consequently extends the G1/swarmer cell lifetime of C. crescentus. (A–D) Extension of the G1/swarmer lifetime in spoT_ΔACTcells is suppressed by inactivating ptsP (encoding EI^Ntr). Motility (A), DNA content (B) and growth (C) were measured in WT (RH50), ΔspoT (RH1755), spoT_D81G(RH1752), ΔptsP (RH1758), spoT_ΔACT (RH1476) and ΔptsP spoT_ΔACT(RH1478) strains grown in complex media PYE. (D) Data shown in a-c were normalized to the WT (100%). Error bars = SD, n = 3. (E) Inactivation of the ACT domain leads to (p)ppGpp accumulation. The intracellular levels of (p)ppGpp were evaluated by TLC after nucleotides extraction from the same strains grown in nitrogen-replete (+N) conditions.