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TbMCM-BP is required for DNA replication. (A) BrdU pulse to label replicating cells. WT and TbMCM-BP cKO cells treated with tetracycline for 0, 1 and 2 days were pulse labeled with 500 μM BrdU for 40 min. BrdU incorporation was detected using anti-BrdU antibody. BrdU pulse labeled cells were stained with Propidium Iodide (PI) and analyzed by flow cytometry. (B) Cell cycle profile with PI channel only.
