Figure 2.

TbMCM-BP is required for DNA replication initiation at early firing origins. Replication was examined by MFA-seq. Cells were collected at day 0 and 1 after tetracycline addition and stained with PI. Cells in G1, early S, late S and G2 phase were sorted by flow cytometry (Supplementary Figure S2), genomic DNA was isolated and high-throughput sequenced. To examine replication profiles, chromosomes were binned at 10 kb resolution with 2.5 kb step. Read count ratio between early S to G1 was plotted for 11 megabase chromosomes. Chromosomes are depicted as bars above each graph. Blue genes are transcribed reverse and red are forward direction. Grey lines indicate the early replicating origins previously identified by the McCulloch lab [7]. Green arrows indicate locations of centromere.