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Effect of RPA in pre-staged reactions. D-loop formation by Dmc1 and its accessory proteins was examined using 90C ssDNA and 90NF ssDNA (30 nM or 2.7 μM nt). Reactions were with or without RPA as indicated. Abbreviations and reaction component concentrations were as in Figure 1 (n = 3, ±s.e.m.).
